Gene Synthesis Service with Free Codon Optimization

Since our first gene was synthesized back in 2004, Epoch Life Science (originally Epoch Biolabs) has evolved to become one of the most dedicated gene synthesis companies. A plethora of references from peer-reviewed journals as well as patents, see below, certainly tells you more about our capability. Every project is taken seriously at Epoch with a focus on accuracy, speed and minimum cost. Our PhD experts go extra miles to help you design your projects with their extensive expertise in protein biochemistry, structural biology, cell biology and molecular biology. Minimizing your risk of wasting money, more importantly, saving your time and efforts in reaching your final goals. Our dedication can not always be matched elsewhere. Epoch Life Science can accommodate hundreds of projects simultaneously with hundreds of well trained associates. High GC, hairpin, tandem repeats will not deter us. Your gene smith can make any gene you can dream of. We are here to accelerate your research.

Detailed Specifications:

• Full Service: Cloned into our standard vector pBlueScript or pUC and sequence verified. Minimum order for each small gene is $150.00. Guaranteed low price, email or call for quote. 
  
• Optional codon optimization with no extra charges are included for both services.
• Single-host vs. multi-host co-don optimization for over-expression 
• Complete sequencing* (for full service only), 100% accuracy
• As fast as 6 days, on average 2-3 week turn around time**

Turn Around Time:

* Under 7 business days for synthetic fragments. Full Serviced (cloned and sequence verified) are as follows:
	Gene size	Average Turn Around Time***
	< 1kp	6-12 business days
	1-3kb	10-17 business days
	3-10kb	15-25 business days

Gene Variant:

	Gene size	Average Turn Around Time***
	< 1kp	6 business days
	1-3kb	6 business days
	3-10kb	6 business days
* Only when ordered together with gene synthesis

Custom Cloning:

	Service	Average Turn Around Time***
	Subcloning	6 business days
	cDNA Clone	6 business days
	Knockout/KnockIn	6 business days

 

For a non binding quote of our Full Service for your particular project, email your sequence to gene@epochlifescience.com

Other Epoch Life Science Services You might be interested in:

Custom Cloning                    Site-Directed Mutagenesis                 DNA Sequencing          Protein Expression Optimization

 

References citing our gene synthesis services:

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Alder, A., I. Holdermann, P. Beyer, and S. Al-Babili. "Carotenoid Oxygenases Involved in Plant Branching Catalyse a Highly Specific Conserved Apocarotenoid Cleavage Reaction." Biochem. J 416, (2008): 289-296.

Alvarez, J.P., I. Pekker, A. Goldshmidt, E. Blum, Z. Amsellem, and Y. Eshed. "Endogenous and Synthetic Micrornas Stimulate Simultaneous, Efficient, and Localized Regulation of Multiple Targets in Diverse Species." The Plant Cell Online 18, no. 5 (2006): 1134-1151.

Amanda, J., J.M. Hyser, V.V. Keasler, Y. Cang, and B.L. Slagle. "Hepatitis B Virus Regulatory Hbx Protein Binding to Ddb1 Is Required but Is Not Sufficient for Maximal Hbv Replication." Virology, (2012).

Antonczak, A.K., Z. Simova, and E.M. Tippmann. "A Critical Examination of Escherichia Coli Esterase Activity." Journal of Biological Chemistry 284, no. 42 (2009): 28795-28800.

Antonczak, A.K., Z. Simova, I.T. Yonemoto, M. Bochtler, A. Piasecka, H. Czapińska, A. Brancale, and E.M. Tippmann. "Importance of Single Molecular Determinants in the Fidelity of Expanded Genetic Codes." Proceedings of the National Academy of Sciences 108, no. 4 (2011): 1320-1325.

Araújo, J.A. "Produção De Quimosina B De Bos Taurus Em Pichia Pastoris." (2008).

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Avedillo Diez, I., D. Zychlinski, E.G. Coci, M. Galla, U. Modlich, R.A. Dewey, A. Schwarzer, T. Maetzig, N. Mpofu, and E. Jaeckel. "Development of Novel Efficient Sin Vectors with Improved Safety Features for Wiskott-Aldrich Syndrome Stem Cell Based Gene Therapy." Molecular pharmaceutics, (2011).

Azizi, A., A. Arora, A. Markiv, D.J. Lampe, T. Miller, and A.S. Kang. "Ribosome Display of Combinatorial Antibody Libraries Derived from Mice Immunised with Heat-Killed Xylella Fastidiosa and the Selection of Mopb-Specific Single-Chain Antibodies." Applied and environmental microbiology, (2012).

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CUNHA, C.E.P., P.L. TELMO, and F.R. CONCEIÇÃO. "Clonagem E Expressão Do Antígeno De Excreção E Secreção Tes-30 De Toxocara Canis Em Escherichia Coli."

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D'Aoust, M.A., P.O. Lavoie, M.M.J. Couture, S. Trépanier, J.M. Guay, M. Dargis, S. Mongrand, N. Landry, B.J. Ward, and L.P. Vézina. "Influenza Virus‐Like Particles Produced by Transient Expression in Nicotiana Benthamiana Induce a Protective Immune Response against a Lethal Viral Challenge in Mice." Plant biotechnology journal 6, no. 9 (2008): 930-940.

Davis, R.W., J.A. Timlin, J.N. Kaiser, M.B. Sinclair, HD Jones, and T.W. Lane. "Accurate Detection of Low Levels of Fluorescence Emission in Autofluorescent Background: Francisella-Infected Macrophage Cells." Microscopy and Microanalysis 16, no. 4 (2010): 478-487.

De Breyne, S., Y. Yu, T.V. Pestova, and C.U.T. Hellen. "Factor Requirements for Translation Initiation on the Simian Picornavirus Internal Ribosomal Entry Site." Rna 14, no. 2 (2008): 367-380.

DE ESTROGÊNIO, P.D.O.S.R.H., and E.P. EM. "Produção Dos Receptores Humanos De Estrogênio E Progesterona Em Escherichia Coli Santos Junior, Alceu Gonçalves1; Aleixo, José Antonio Guimarães2; Conceição, Fabrício Rochedo3."

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Diao, F., and B.H. White. "A Novel Approach for Directing Transgene Expression in Drosophila: T2a-Gal4 in-Frame Fusion." Genetics, (2011).

Dinh, T., and T.G. Bernhardt. "Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies." Journal of bacteriology 193, no. 18 (2011): 4984-4987.

Evans, R.M., E.M. Behiry, L.H. Tey, J. Guo, E.J. Loveridge, and R.K. Allemann. "Catalysis by Dihydrofolate Reductase from the Psychropiezophile Moritella Profunda." ChemBioChem 11, no. 14 (2010).

Franz, AWE, N. Jasinskiene, I. Sanchez‐Vargas, AT Isaacs, MR Smith, CCH Khoo, MS Heersink, AA James, and KE Olson. "Comparison of Transgene Expression in Aedes Aegypti Generated by Mariner Mos1 Transposition and Φc31 Site‐Directed Recombination." Insect Molecular Biology, (2011).

Galazka, J., B. Striepen, and B. Ullman. "Adenosine Kinase from< I> Cryptosporidium Parvum</I>." Molecular and biochemical parasitology 149, no. 2 (2006): 223-230.

Guillaumond, F., A. Gréchez-Cassiau, M. Subramaniam, S. Brangolo, B. Peteri-Brünback, B. Staels, C. Fiévet, T.C. Spelsberg, F. Delaunay, and M. Teboul. "Krüppel-Like Factor Klf10 Is a Link between the Circadian Clock and Metabolism in Liver." Molecular and cellular biology 30, no. 12 (2010): 3059-3070.

Gurda, B.L., K.N. Parent, H. Bladek, R.S. Sinkovits, M.A. DiMattia, C. Rence, A. Castro, R. McKenna, N. Olson, and K. Brown. "Human Bocavirus Capsid Structure: Insights into the Structural Repertoire of the Parvoviridae." Journal of virology 84, no. 12 (2010): 5880-5889.

Han, X., and E.S. Boyden. "Multiple-Color Optical Activation, Silencing, and Desynchronization of Neural Activity, with Single-Spike Temporal Resolution." PLoS One 2, no. 3 (2007): e299.

Hanai, T., S. Atsumi, and JC Liao. "Engineered Synthetic Pathway for Isopropanol Production in Escherichia Coli." Applied and environmental microbiology 73, no. 24 (2007): 7814-7818.

Harris, B., I. Pereira, and E. Parkin. "Targeting Adam10 to Lipid Rafts in Neuroblastoma Sh-Sy5y Cells Impairs Amyloidogenic Processing of the Amyloid Precursor Protein." Brain research 1296, (2009): 203-215.

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Isaacs, A.T., F. Li, N. Jasinskiene, X. Chen, X. Nirmala, O. Marinotti, J.M. Vinetz, and A.A. James. "Engineered Resistance to Plasmodium Falciparum Development in Transgenic Anopheles Stephensi." PLoS pathogens 7, no. 4 (2011): e1002017.

Jack, B.H.A., and M. Crossley. "Gata Proteins Work Together with Friend of Gata (Fog) and C-Terminal Binding Protein (Ctbp) Co-Regulators to Control Adipogenesis." Journal of Biological Chemistry 285, no. 42 (2010): 32405-32414.

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Janovjak, H., S. Szobota, C. Wyart, D. Trauner, and E.Y. Isacoff. "A Light-Gated, Potassium-Selective Glutamate Receptor for the Optical Inhibition of Neuronal Firing." Nature neuroscience 13, no. 8 (2010): 1027-1032.

Keasling, J.D., Y. Kang, E.J. Steen, and G. Bokinsky. "Product of Fatty Acid Esters from Biomass Polymers." US Patent 20,120,115,195, 2012.

Kirby, K.A., B. Marchand, Y.T. Ong, T.P. Ndongwe, A. Hachiya, E. Michailidis, M.D. Leslie, D.V. Sietsema, T.L. Fetterly, and C.A. Dorst. "Structural and Inhibition Studies of the Rnase H Function of Xenotropic Murine Leukemia Virus-Related Virus Reverse Transcriptase." Antimicrobial Agents and Chemotherapy, (2012).

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Le Brun, A.P., D.S.H. Shah, D. Athey, S.A. Holt, and J.H. Lakey. "Self-Assembly of Protein Monolayers Engineered for Improved Monoclonal Immunoglobulin G Binding." International journal of molecular sciences 12, no. 8 (2011): 5157-5167.

Lenormand, C., H. Bausinger, F. Gross, F. Signorino-Gelo, S. Koch, M. Peressin, D. Fricker, J.P. Cazenave, T. Bieber, and D. Hanau. "Hla-Dqa2 and Hla-Dqb2 Genes Are Specifically Expressed in Human Langerhans Cells and Encode a New Hla Class Ii Molecule." The Journal of Immunology 188, no. 8 (2012): 3903-3911.

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Liew, P.X., C.L.C. Wang, and S.L. Wong. "Functional Characterization and Localization of a Bacillus Subtilis Sortase and Its Substrate and Use of This Sortase System to Covalently Anchor a Heterologous Protein to the B. Subtilis Cell Wall for Surface Display." Journal of bacteriology 194, no. 1 (2012): 161-175.

Lim, C.G. "Metabolic Engineering for High-Level Flavonoids and Stilbenes Production in Microorganisms." STATE UNIVERSITY OF NEW YORK AT BUFFALO, 2012.

Lim, C.G., Z.L. Fowler, T. Hueller, S. Schaffer, and M.A.G. Koffas. "High-Yield Resveratrol Production in Engineered Escherichia Coli." Applied and environmental microbiology 77, no. 10 (2011): 3451-3460.

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Ma, M., Z.L. Liu, and J. Moon. "Genetic Engineering of Inhibitor-Tolerant Saccharomyces Cerevisiae for Improved Xylose Utilization in Ethanol Production." BioEnergy Research, (2012): 1-11.

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Markiv, A., B. Anani, R.V. Durvasula, and A.S. Kang. "Module Based Antibody Engineering: A Novel Synthetic Redantibody." Journal of immunological methods 364, no. 1 (2011): 40-49.

Markiv, A., R. Beatson, J. Burchell, R.V. Durvasula, and A.S. Kang. "Expression of Recombinant Multi-Coloured Fluorescent Antibodies in Gor-/Trxb-E. Coli Cytoplasm." BMC biotechnology 11, no. 1 (2011): 117.
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Michael, D.R., A.O. Phillips, A. Krupa, J. Martin, J.E. Redman, A. Altaher, R.D. Neville, J. Webber, M. Kim, and T. Bowen. "The Human Hyaluronan Synthase 2 (Has2) Gene and Its Natural Antisense Rna Exhibit Coordinated Expression in the Renal Proximal Tubular Epithelial Cell." Journal of Biological Chemistry 286, no. 22 (2011): 19523.

Moreira, S., F. Andrade, L. Domingues, and M. Gama. "Development of a Strategy to Functionalize a Dextrin-Based Hydrogel for Animal Cell Cultures Using a Starch-Binding Module Fused to Rgd Sequence." BMC biotechnology 8, no. 1 (2008): 78.

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Naesby, M., S.V.S. Nielsen, C.A.F. Nielsen, T. Green, T.Ø. Tange, E. Simón, P. Knechtle, A. Hansson, M.S. Schwab, and O. Titiz. "Yeast Artificial Chromosomes Employed for Random Assembly of Biosynthetic Pathways and Production of Diverse Compounds in Saccharomyces Cerevisiae." Microbial cell factories 8, no. 1 (2009): 45.

Ndongwe, T.P., A.O. Adedeji, E. Michailidis, Y.T. Ong, A. Hachiya, B. Marchand, E.M. Ryan, D.K. Rai, K.A. Kirby, and A.S. Whatley. "Biochemical, Inhibition and Inhibitor Resistance Studies of Xenotropic Murine Leukemia Virus-Related Virus Reverse Transcriptase." Nucleic acids research 40, no. 1 (2012): 345-359.

Neuteboom, L.W., K.O. Matsumoto, and D.A. Christopher. "An Extended Ae-Rich N-Terminal Trunk in Secreted Pineapple Cystatin Enhances Inhibition of Fruit Bromelain and Is Posttranslationally Removed During Ripening." Plant physiology 151, no. 2 (2009): 515-527.

Nunes, A.C.S. "Biofortificação De Plantas De Alface (Lactuca Sativa L.) Geneticamente Modificadas Para Aumento Do Teor De Folato." (2010).

Nunes, A.C.S., D.C. Kalkmann, and F.J.L. Aragao. "Folate Biofortification of Lettuce by Expression of a Codon Optimized Chicken Gtp Cyclohydrolase I Gene." Transgenic research 18, no. 5 (2009): 661-667.

O'Connor, T.P., J.M. Saucier, D. Daniluk, B.A. Stillman, R. Krah, Y. Rikihisa, Q. Xiong, M.J. Yabsley, D.S. Adams, and P.P.V.P. Diniz. "Evaluation of Peptide-and Recombinant Protein-Based Assays for Detection of Anti-Ehrlichia Ewingii Antibodies in Experimentally and Naturally Infected Dogs." American journal of veterinary research 71, no. 10 (2010): 1195-1200.

O'Sullivan, V.J., I. Barrette-Ng, E. Hommema, G.T. Hermanson, M. Schofield, S.C. Wu, C. Honetschlaeger, K.K.S. Ng, and S.L. Wong. "Development of a Tetrameric Streptavidin Mutein with Reversible Biotin Binding Capability: Engineering a Mobile Loop as an Exit Door for Biotin." PLoS One 7, no. 4 (2012): e35203.

Oka, T., E. Remue, K. Meerschaert, B. Vanloo, C. Boucherie, D. Gfeller, G. Bader, S. Sidhu, J. Vandekerckhove, and J. Gettemans. "Functional Complexes between Yap2 and Zo-2 Are Pdz Domain-Dependent, and Regulate Yap2 Nuclear Localization and Signalling1." Biochem. J 432, (2010): 461-472.

Parkin, E., and B. Harris. "A Disintegrin and Metalloproteinase (Adam)‐Mediated Ectodomain Shedding of Adam10." Journal of neurochemistry 108, no. 6 (2009): 1464-1479.

Parkin, E.T., C.A. Parr-Sturgess, and D.J. Rushton. "Ectodomain Shedding of the Notch Ligand Jagged1 Is Mediated by Adam17 but Is Not a Lipid Raft-Associated Event." (2010).

Parra, G., K. Bradnam, A.B. Rose, and I. Korf. "Comparative and Functional Analysis of Intron-Mediated Enhancement Signals Reveals Conserved Features among Plants." Nucleic acids research 39, no. 13 (2011): 5328-5337.

Péréfarres, F., M. Hoareau, F. Chiroleu, B. Reynaud, J. Dintinger, and J.M. Lett. "A Novel Synthetic Quantification Standard Including Virus and Internal Report Targets: Application for the Detection and Quantification of Emerging Begomoviruses on Tomato." Virology Journal 8, no. 1 (2011): 389.

PEREIRA, J.L., G.B. KLAFKE, G. MOREIRA, L.S. PINTO, F.R. CONCEIÇÃO, and F.P. LEITE. "Clonagem E Expressão Da Quimera Ltb/Gnrh Em Pichia Pastoris."

Perez-Jimenez, R., A. Inglés-Prieto, Z.M. Zhao, I. Sanchez-Romero, J. Alegre-Cebollada, P. Kosuri, S. Garcia-Manyes, T.J. Kappock, M. Tanokura, and A. Holmgren. "Single-Molecule Paleoenzymology Probes the Chemistry of Resurrected Enzymes." Nature structural & molecular biology 18, no. 5 (2011): 592-596.

Pierre, M., J.A. Traverso, B. Boisson, S. Domenichini, D. Bouchez, C. Giglione, and T. Meinnel. "N-Myristoylation Regulates the Snrk1 Pathway in Arabidopsis." The Plant Cell Online 19, no. 9 (2007): 2804-2821.

Pisarev, A.V., A. Unbehaun, C.U.T. Hellen, and T.V. Pestova. "Assembly and Analysis of Eukaryotic Translation Initiation Complexes." Methods in enzymology 430, (2007): 147-177.

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