[shortdesc]Silica membrane spin column based total isolation kit for the isolation of RNA molecules larger than 200 nucleotides. Two sizes to choose from: 50 preps or 250 preps per kit.[/shortdesc]

[additional]Product 001 Additional Content Here [/additional]

[els_overview]GenCatch™ Total RNA Extraction Miniprep System provides an economical method to purify total RNA from various samples such as cultured cells, tissues, and bacteria. A simple silica-membrane spin-column method can isolate total RNA without need of performing time-consuming phenol/ chloroform extraction and ethanol precipitation. Total RNA longer than 200 nucleotides are isolated, while small RNA such as 5.8S RNA, 5S RNA, and tRNA, which make up 15-20% of the total RNA, are excluded.

Sample Size:

Samples	Recommended Amount of Samples		Yield (µg)
Animal Cells	NIH-3T3	1 x 10⁶cells	12
	HeLa	1 x 10⁶cells	15
	COS-7	1 x 10⁶cells	30
	LMH	1 x 10⁶cells	12
Animal Tissues	Mouse/rat tissues
	Embryo	10 mg	30
	Heart	10 mg	10
	Brain	10 mg	10
	Kidney	10 mg	35
	Liver	10 mg	45
	Spleen	10 mg	35
	Lung	10 mg	10
	Thymus	10 mg	45
Bacteria	E.coli	1 x 10⁹ cells	45
	B. subtilis	1 x 10⁹ cells	40

Yield: Up to 100 μg/per column of total RNA.

Quality: RNA purified is suitable for many downstream molecular manipulations including, RT-PCR, cDNA synthesis, in vitro translation, etc.

Convenient: No phenol extractions or ethanol precipitations required.

GenCatch™ Total RNA Mini Prep Flow Chart:

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	Cat. No.: 1660050	Cat. No.: 1660250
RX Buffer	36 ml	150 ml
WF Buffer	30 ml	150 ml
WS Buffer	15 ml	45 ml
RNase-free ddH2O	3 ml	25 ml
GenCatch™ Mini Column	50 pieces	250 pieces
Collection Tube	50 pieces	250 pieces
Elution Tube	50 pieces	250 pieces

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GenCatch™ Total RNA Miniprep Protocol:

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[els_sds] Will be uploaded soon, thanks for your patience [/els_sds]

[els_citations]Click the links below to learn about recent publications citing our products from Google Scholar:

Epoch Biolabs (our old name)

Epoch Life Science (our correct name)

Epoch Life Sciences (typo by some authors)

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GenCatch™ Total RNA Mini Prep Kit