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Silica membrane spin column based total isolation kit for the isolation of RNA molecules larger than 200 nucleotides. Two sizes to choose from: 50 preps or 250 preps per kit.
GenCatch™ Total RNA Extraction Miniprep System provides an economical method to purify total RNA from various samples such as cultured cells, tissues, and bacteria. A simple silica-membrane spin-column method can isolate total RNA without need of performing time-consuming phenol/ chloroform extraction and ethanol precipitation. Total RNA longer than 200 nucleotides are isolated, while small RNA such as 5.8S RNA, 5S RNA, and tRNA, which make up 15-20% of the total RNA, are excluded.
Sample Size:
| Samples | Recommended Amount of Samples | Yield (µg) | |
| Animal Cells | NIH-3T3 | 1 x 106cells | 12 |
| HeLa | 1 x 106cells | 15 | |
| COS-7 | 1 x 106cells | 30 | |
| LMH | 1 x 106cells | 12 | |
| Animal Tissues | Mouse/rat tissues | ||
| Embryo | 10 mg | 30 | |
| Heart | 10 mg | 10 | |
| Brain | 10 mg | 10 | |
| Kidney | 10 mg | 35 | |
| Liver | 10 mg | 45 | |
| Spleen | 10 mg | 35 | |
| Lung | 10 mg | 10 | |
| Thymus | 10 mg | 45 | |
| Bacteria | E.coli | 1 x 109 cells | 45 |
| B. subtilis | 1 x 109 cells | 40 | |
Yield: Up to 100 μg/per column of total RNA.
Quality: RNA purified is suitable for many downstream molecular manipulations including, RT-PCR, cDNA synthesis, in vitro translation, etc.
Convenient: No phenol extractions or ethanol precipitations required.
GenCatch™ Total RNA Mini Prep Flow Chart:
| Cat. No.: 1660050 | Cat. No.: 1660250 | |
| RX Buffer | 36 ml | 150 ml |
| WF Buffer | 30 ml | 150 ml |
| WS Buffer | 15 ml | 45 ml |
| RNase-free ddH2O | 3 ml | 25 ml |
| GenCatch™ Mini Column | 50 pieces | 250 pieces |
| Collection Tube | 50 pieces | 250 pieces |
| Elution Tube | 50 pieces | 250 pieces |
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GenCatch™ Total RNA Miniprep Protocol:
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