[shortdesc] Standard silica membrane/chaotropic salt based method. Average yield of 1-20 ug plasmid DNA from 1-3 ml overnight bacterial culture. Two sizes to choose from: 50 preps or 250 preps per kit.[/shortdesc]

[els_overview]GenCatch™ Plasmid DNA Mini Prep Kit provides a simple, fast and cost-effective method to purify plasmid DNA without phenol/chloroform extraction. It is based on binding of DNA to silica-based membranes in a chaotropic salt. An average yield of 1-20 ug plasmid DNA can be expected from 1-3 ml overnight bacterial culture.

Fast: Up to 24 samples can be readily prepared in 30 minutes with minimum efforts.

Flexible: Mini columns can used in either centrifugation procedure or using a vacuum manifold.

Convenient: No phenol extractions or ethanol precipitations required.

Yield: 4-20 μg of DNA from 2 ml of culture

Quality: DNA prepared by this kit is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing.

GenCatch™ Plasmid Mini Prep Flow Chart:

Sequencing result of plasmid pBluescriptSK purified by GenCatch Plasmid DNA Miniprep Kit using ABI 3730xl sequencer:

Sequencing Result from Mini Prep

Plasmid DNA ranging from 4 to 12 kb purified from E. coli DH5a with GenCatch Plasmid DNA Miniprep Kit and run on 1% agarose gel:
DNA Mini Prep

Restriction enzyme digestion of pBluescriptSK plasmid purified by GenCatch Plasmid DNA Miniprep Kit and analyzed on a 1.5% agarose gel:

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[els_components]

	Cat. No.: 2160050	Cat. No.: 2160250
MX1 Buffer	12 ml	60 ml
MX2 Buffer	15 ml	75 ml
MX3 Buffer	20 ml	100 ml
WN Buffer	6 ml	45 ml
WS Buffer	10 ml	50 ml
Elution Buffer	5 ml	25 ml
RNase A 20 mg/ml	42 µl	210 µl
GenCatch™ Mini Column	50 pieces	250 pieces
Collection Tube	50 pieces	250 pieces

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[els_protocol]

GenCatch™ Plasmid DNA Mini Prep Protocol:

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[els_sds] Will be uploaded soon, thanks for your patience [/els_sds]

[els_citations]Click the links below to learn about recent publications citing our products from Google Scholar:

Epoch Biolabs (our old name)

Epoch Life Science (our correct name)

Epoch Life Sciences (typo by some authors)

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GenCatch™ Plasmid DNA Mini-Prep Kit