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Standard silica membrane/chaotropic salt based method. Average yield of 1-20 ug plasmid DNA from 1-3 ml overnight bacterial culture. Two sizes to choose from: 50 preps or 250 preps per kit.
GenCatch™ Plasmid DNA Mini Prep Kit provides a simple, fast and cost-effective method to purify plasmid DNA without phenol/chloroform extraction. It is based on binding of DNA to silica-based membranes in a chaotropic salt. An average yield of 1-20 ug plasmid DNA can be expected from 1-3 ml overnight bacterial culture.
Fast: Up to 24 samples can be readily prepared in 30 minutes with minimum efforts.
Flexible: Mini columns can used in either centrifugation procedure or using a vacuum manifold.
Convenient: No phenol extractions or ethanol precipitations required.
Yield: 4-20 μg of DNA from 2 ml of culture
Quality: DNA prepared by this kit is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing.
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GenCatch™ Plasmid Mini Prep Flow Chart: 
Sequencing result of plasmid pBluescriptSK purified by GenCatch Plasmid DNA Miniprep Kit using ABI 3730xl sequencer:
Plasmid DNA ranging from 4 to 12 kb purified from E. coli DH5a with GenCatch Plasmid DNA Miniprep Kit and run on 1% agarose gel: |
| Cat. No.: 2160050 | Cat. No.: 2160250 | |
| MX1 Buffer | 12 ml | 60 ml |
| MX2 Buffer | 15 ml | 75 ml |
| MX3 Buffer | 20 ml | 100 ml |
| WN Buffer | 6 ml | 45 ml |
| WS Buffer | 10 ml | 50 ml |
| Elution Buffer | 5 ml | 25 ml |
| RNase A 20 mg/ml | 42 µl | 210 µl |
| GenCatch™ Mini Column | 50 pieces | 250 pieces |
| Collection Tube | 50 pieces | 250 pieces |
GenCatch™ Plasmid DNA Mini Prep Protocol:
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Epoch Life Science (our correct name)
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