[shortdesc] For purification of PCR fragments from 70bp to 10 kb. Silica membrane spin column based. Two sizes to choose from: 50 preps or 250 preps per kit.[/shortdesc]

[additional]Product 001 Additional Content Here [/additional]

[els_overview]GenCatch™ PCR Cleanup Kit purifies DNA molecules from PCR and other enzymatic reactions from enzymes, dNTPs, salts, and primers without phenol/chloroform extraction. Recovery efficiency of DNA fragments ranging from 70 bp to 10 kb in size is up to 95%. It totally eliminate slurry carryover problem as encountered using silica beads or glass milk based methods. Up to 10 µg DNA can be recovered with a single column and elution volumes can be as small as 10 µl.

Capacity: Up to 10 µg of DNA.

Elution Volume: 10-50 µl

Recover Efficiency: Up to 95%.

Quality: DNA purified using GenCatch™ PCR Cleanup Kit is free from agraose, carry-over slurry and salt contamination. And is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing.

Fast: Single samples can be prepared within 10 minutes with minimum efforts. 18-24 samples can be simultaneously processed in a centrifugation format within 30 minutes. Or, more can be processed using a vacuum manifold.

Flexible: Mini columns can used in either centrifugation procedure or using a vacuum manifold.

Convenient: No phenol extractions or ethanol precipitations required.

GenCatch™ PCR Cleanup Flow Chart:

PCR fragments of various MW before (lane 1-5) were purified using GenCatch™ PCR Clenup Kit and loaded as cocktail:

PCR fragment purification

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[els_components]

	Cat. No.: 2360050	Cat. No.: 2360250
PX Buffer	30 ml	150 ml
WN Buffer	6 ml	30 ml
WS Buffer	6 ml	30 ml
Elution Buffer	5 ml	25 ml
GenCatch™ Mini Column	50 pieces	250 pieces
Collection Tube	50 pieces	250 pieces

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[els_protocol]

GenCatch™ PCR Cleanup Protocol:

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[els_sds] Will be uploaded soon, thanks for your patience [/els_sds]

[els_citations]Click the links below to learn about recent publications citing our products from Google Scholar:

Epoch Biolabs (our old name)

Epoch Life Science (our correct name)

Epoch Life Sciences (typo by some authors)

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