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For purification of PCR fragments from 70bp to 10 kb. Silica membrane spin column based. Two sizes to choose from: 50 preps or 250 preps per kit.
GenCatch™ PCR Cleanup Kit purifies DNA molecules from PCR and other enzymatic reactions from enzymes, dNTPs, salts, and primers without phenol/chloroform extraction. Recovery efficiency of DNA fragments ranging from 70 bp to 10 kb in size is up to 95%. It totally eliminate slurry carryover problem as encountered using silica beads or glass milk based methods. Up to 10 µg DNA can be recovered with a single column and elution volumes can be as small as 10 µl.
Capacity: Up to 10 µg of DNA.
Elution Volume: 10-50 µl
Recover Efficiency: Up to 95%.
Quality: DNA purified using GenCatch™ PCR Cleanup Kit is free from agraose, carry-over slurry and salt contamination. And is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing.
Fast: Single samples can be prepared within 10 minutes with minimum efforts. 18-24 samples can be simultaneously processed in a centrifugation format within 30 minutes. Or, more can be processed using a vacuum manifold.
Flexible: Mini columns can used in either centrifugation procedure or using a vacuum manifold.
Convenient: No phenol extractions or ethanol precipitations required.
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GenCatch™ PCR Cleanup Flow Chart: 
PCR fragments of various MW before (lane 1-5) were purified using GenCatch™ PCR Clenup Kit and loaded as cocktail:
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| Cat. No.: 2360050 | Cat. No.: 2360250 | |
| PX Buffer | 30 ml | 150 ml |
| WN Buffer | 6 ml | 30 ml |
| WS Buffer | 6 ml | 30 ml |
| Elution Buffer | 5 ml | 25 ml |
| GenCatch™ Mini Column | 50 pieces | 250 pieces |
| Collection Tube | 50 pieces | 250 pieces |
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GenCatch™ PCR Cleanup Protocol:
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