Codon Optimization




We offer codon optimization as a complimentary service with our gene synthesis service. However, if you prefer not to use our gene synthesis service but to take advantage of our advance codon optimization algorithms, we would still be delighted to assist.

Codon optimization is a process of modifying the nucleotide sequence of a gene to improve its expression in a specific host organism. This is done by replacing rare codons, which are less frequently used by the host organism, with synonymous codons that are more frequently used.

The application of codon optimization is mainly seen in recombinant protein expression. Recombinant proteins are typically produced in a heterologous host organism, such as bacteria, yeast, or insect cells. However, the codon usage of the target gene may not be optimized for the host organism, resulting in poor expression levels or even non-expression of the protein of interest. Codon optimization can enhance protein expression levels by increasing the efficiency of translation and reducing ribosomal stalling, premature termination, and protein misfolding.

At Epoch Life Science, we understand that gene expression is a complex process and that the choice of codons can significantly impact the efficiency of protein expression. Our codon optimization service uses advanced algorithms to identify the better adapted sequence for a particular host organism, resulting in increased protein expression levels.

With our codon optimization service, you can expect:

  • Increased protein expression levels
  • Improved solubility and stability of proteins
  • Reduced likelihood of protein misfolding and aggregation


Our team of experts has extensive experience in codon optimization for a wide range of organisms, including insect cell, bacteria, yeast, and mammalian cells. We use the latest software tools and databases to ensure that your gene is optimized for maximum expression and functionality.

Figure Above: Codon optimization for E.coli expression.
Lane 1, Total cell extract of uninduced blank vector control; Lane 2, Total cell extract from un-optimized wild-type gene; Lane 3, Total cell extract from codon optimized gene;  Lane 4, Supernatant of uninduced blank vector control; Lane 5, Supernatant from un-optimized gene; Lane 6, Supernatant from codon optimized gene. Target protein is ~60 kD.


Figure Below: Codon optimization for HEK293 cell expression.
10 randomly selected optimized sequences, together with the un-optimized wild-type gene were synthesized and expressed with His-tag in HEK293 cell. Protein expression level was measured using His-tag specific ELISA with serial diluted samples.

Whether you need to express a single gene or an entire pathway, our codon optimization service can help you achieve the results you need. We offer affordable prices, fast turnaround times, and high-quality service. Contact us today to learn more about how we can help you optimize your gene expression and achieve your research goals!

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