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Silica membrane spin column based total isolation kit for the isolation of RNA molecules larger than 200 nucleotides. Two sizes to choose from: 50 preps or 250 preps per kit.
GenCatch™ Total RNA Extraction Miniprep System provides an economical method to purify total RNA from various samples such as cultured cells, tissues, and bacteria. A simple silica-membrane spin-column method can isolate total RNA without need of performing time-consuming phenol/ chloroform extraction and ethanol precipitation. Total RNA longer than 200 nucleotides are isolated, while small RNA such as 5.8S RNA, 5S RNA, and tRNA, which make up 15-20% of the total RNA, are excluded.
|Samples||Recommended Amount of Samples||Yield (µg)|
|Animal Cells||NIH-3T3||1 x 106cells||12|
|HeLa||1 x 106cells||15|
|COS-7||1 x 106cells||30|
|LMH||1 x 106cells||12|
|Animal Tissues||Mouse/rat tissues|
|Bacteria||E.coli||1 x 109 cells||45|
|B. subtilis||1 x 109 cells||40|
Yield: Up to 100 μg/per column of total RNA.
Quality: RNA purified is suitable for many downstream molecular manipulations including, RT-PCR, cDNA synthesis, in vitro translation, etc.
Convenient: No phenol extractions or ethanol precipitations required.
GenCatch™ Total RNA Mini Prep Flow Chart:
|Cat. No.: 1660050||Cat. No.: 1660250|
|RX Buffer||36 ml||150 ml|
|WF Buffer||30 ml||150 ml|
|WS Buffer||15 ml||45 ml|
|RNase-free ddH2O||3 ml||25 ml|
|GenCatch™ Mini Column||50 pieces||250 pieces|
|Collection Tube||50 pieces||250 pieces|
|Elution Tube||50 pieces||250 pieces|
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