SeqPure™ Sanger Sequencing Cleanup Resin

SeqPure™ is a unique dual-function resin engineered for efficient removal of sequencing contaminants while preserving fluorescently labeled extension products in solution.

SeqPure™ Resin incorporates:

• An ion-exchange core designed to capture charged small-molecule contaminants
• A size-exclusion outer layer that selectively limits access based on molecular size

This architecture enables preferential sequestration of unwanted reaction components including:

• Unincorporated dye terminators
• Residual salts and buffer components
• Small charged impurities

At the same time, the larger fluorescent labeled ssDNA fragments remain suspended in the supernatant and are immediately available for electrokinetic injection.

By minimizing loss of sequencing products during cleanup, SeqPure™ helps maintain strong signal intensity while reducing background noise and dye artifacts that can interfere with electropherogram interpretation.

Unlike precipitation-based methods that rely on recovery and resuspension of DNA pellets, SeqPure™ performs cleanup directly in solution, simplifying workflow and improving reproducibility across samples. The result is a rapid and efficient purification process optimized for modern Sanger sequencing workflows.

Key Benefits

Direct Drop-In Replacement

SeqPure™ integrates seamlessly into existing BigDye™ Xterminator™ workflows with no instrument modifications or protocol redesign required. Compatible with standard sequencing chemistries and capillary electrophoresis platforms.

Clean Signal, Excellent Read Quality

Optimized cleanup chemistry minimizes dye blobs, suppresses background noise, and improves electropherogram clarity for high-confidence sequencing results.

Strong Signal Recovery

SeqPure™ maintains excellent recovery of extension products, producing high signal intensity and long readable sequence lengths.

Lower Cost per Sample

Reduce sequencing cleanup costs without compromising data quality. SeqPure™ provides a cost-effective alternative for high-throughput and routine sequencing applications.

Faster and More Convenient Workflow

Unlike magnetic bead purification or ethanol precipitation methods, SeqPure™ eliminates time-consuming wash steps, magnetic separation, centrifugation, pellet drying, and resuspension procedures.

The simplified workflow helps reduce hands-on time and improves laboratory throughput.

Fig. 1 Electropherogram of the result using SeqPure™. 
 

Fig. 2 Electropherogram of the result using BigDye™ X-terminator™.