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5x Sequencing Buffer is specifically designed for use with the BigDye™ Terminator Cycle Sequencing Kit. It allows further dilution of BigDye™ and is a key component to save on BigDye™ Terminator reagent. It contains a proprietary combination of reagents that help to optimize the Sanger sequencing reaction and improve the accuracy and reliability of the sequencing results.
One of the key features of the 5x sequencing buffer is its high salt concentration. This helps to stabilize the DNA polymerase enzyme and improve its processivity, which in turn leads to more accurate and reliable sequencing results.
Another important aspect of the 5x sequencing buffer is its pH stability. It maintains a stable pH over a wide range of temperatures, which helps to ensure consistent sequencing results even in challenging experimental conditions.
The 5x sequencing buffer is also optimized for use with fluorescently labeled dyes, which are commonly used in capillary electrophoresis sequencing.
Overall, the 5x sequencing buffer is a highly specialized reagent that has been carefully designed and optimized for DNA sequencing applications. Its unique combination of high salt concentration, pH stability, and compatibility with fluorescent dyes make it a valuable tool for researchers and clinicians who need accurate and reliable DNA sequence data.
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