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For the extraction of RNA > 200 nucleotides from plant and fugal samples. Silica membrane mini spin column based method. Two sizes to choose from: 50 preps or 250 preps per kit.
GenCatch™ Plant Total RNA Purification System provides a simple, fast and cost-effective method to purify RNA from plant and fugal samples. It comes with shearing tubes for simple and fast homogenization and filtration of plant tissues. It uses a simple silica-membrane spin-column method to isolate total RNA greater than 200 nucleotides while excluding smaller RNA such as 5S, 5.8S and tRNA without phenol/chloroform extraction and ethanol precipitation. The system also provides additional buffer for handling plant tissue that contai sticky secondary metabolites.
Sample Size: Up to 100 mg plant samples or 1x107 cells.
Quality: RNA isolated can be used for a variety of molecular manipulations such as: Northern blotting, RT-PCR, PolyA+ RNA selection, cDNA synthesis, Primer extension and in vitro translation, etc.
Convenient: No phenol extractions or ethanol precipitations required.
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GenCatch™ Plant RNA Miniprep Flow Chart: 
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| Cat. No.: 1760050 | Cat. No.: 1760250 | |
| RX Buffer | 27 ml | 135 ml |
| PRX Buffer | 27 ml | 135 ml |
| WF Buffer (RNA) |
30 ml | 150 ml |
| WS Buffer | 15 ml | 45 ml x 2 |
| RNase-free ddH2O |
1.5 ml x2 |
15 ml |
| GenCatch™ RNA Mini Column | 50 pieces | 250 pieces |
| Shearing Tube | 50 pieces | 250 pieces |
| Collection Tube | 100 pieces | 500 pieces |
| Elution Tube | 50 pieces | 250 pieces |
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GenCatch™ Plant Total RNA Miniprep Protocol:
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