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For genomic DNA isolation from various samples such as bacteria, yeast, blood, cultured cells, tissue, plasma, serum, buffy coat, lymphocytes, paraffin-embedded tissue and forensic specimens. Two sizes to choose from: 50 preps or 250 preps per kit.
GenCatch™ Blood & Tissue GenomicMiniprep Kit provides a simple, fast and cost-effective method to purify genomic DNA from various samples such as bacteria, yeast, blood, cultured cells, tissue, plasma, serum, buffy coat, lymphocytes, paraffin-embedded tissue and forensic specimens, without phenol/chloroform extraction and ethanol precipitation. Isolated genomic DNA is free of contaminations and are good substrates for southern blotting, enzymatic and PCR reactions.
Sample Size: Up to 5 ml of bacterial or yeast culture, 200 µl of whole blood and 30 mg of animal tissue or culture cells can be processed in a single column.
Yield: Up to 80 µg genomic DNA from bacterial and yeast culture, 120 µg from mammalian tissue culture and 10 µg from whole blood sample can be obtained.
Quality: Genomic DNA purified is suitable for many downstream molecular manipulations including, genotyping, PCR, enzymatic reactions, southern blotting, etc.
Convenient: No phenol extractions or ethanol precipitations required.
GenCatch™ Blood & Tissue Genomic Miniprep Flow Chart:
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Cat. No.: 2460050 | Cat. No.: 2460250 | |
RL Buffer | 100 ml | 250 ml x 2 |
LYS Buffer | 12 ml | 60 ml |
FX Buffer | 17 ml | 85 ml |
WS Buffer | 15 ml | 45 ml x 2 |
Proteinase K | 10 mg | 10 mg x 5 |
GenCatch™ Mini Column | 50 pieces | 250 pieces |
Collection Tube | 100 pieces | 500 pieces |
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