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DEAE anion exchange column based plasmid purification kit for transfection grade plasmid preps
GenCatch™ Plasmid DNA Midi- and Maxi- Prep Kits utilize ion-exchange column to achieve the highest purity of nucleic acids for the most demanding applications, such as mammalian tissue culture transfection. The purification procedure is simple, fast and cost-effective.
Quality: Highest purity using ion-exchange column. DNA prepared by this kit is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing.Quality : Highest purity using ion-exchange column. DNA prepared by this kit is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing.
Convenient: No phenol extractions or ethanol precipitations required.
Yield: 100 µg of DNA for a single Midiprep column and 500 µg DNA for a single Maxiprep column.
GenCatch™ Plasmid DNA Midi-Prep Kit Flow Chart:
Transfection of COS1 cells by 1 µg of pEGFP (left) and pDsRed (right) plasmid DNA purified by GenCatch™ Plasmid DNA Midiprep Kit:
| Cat. No.: 2170025 | Cat. No.: 2170050 | |
| VP1 Buffer | 120 ml | 265 ml |
| VP2 Buffer | 120 ml | 265 ml |
| VP3 Buffer | 120 ml | 265 ml |
| VPN Buffer | 265 ml x 2 | 265 ml x4 |
| VPE Buffer | 130 ml | 265 ml |
| RNase A 20 mg/ml | 0.6 ml | 1.325 ml |
| GenCatch™ Anion-exchange Column | 25 | 50 |
| GenCatch™ Mini Column | 50 | 100 |
GenCatch™ Plasmid DNA Midi Prep Protocol :
Customised SDS Content Here test html
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