DEAE anion exchange column based plasmid purification kit for transfection grade plasmid preps

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GenCatch™ Plasmid DNA Midi- and Maxi- Prep Kits utilize ion-exchange column to achieve the highest purity of nucleic acids for the most demanding applications, such as mammalian tissue culture transfection. The purification procedure is simple, fast and cost-effective.

Quality: Highest purity using ion-exchange column. DNA prepared by this kit is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing.Quality : Highest purity using ion-exchange column. DNA prepared by this kit is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing.

Convenient: No phenol extractions or ethanol precipitations required.

Yield: 100 µg of DNA for a single Midiprep column and 500 µg DNA for a single Maxiprep column.

 

GenCatch™ Plasmid DNA Maxi-Prep Kit Flow Chart:

 

Transfection of COS1 cells by 1 µg of pEGFP (left) and pDsRed (right) plasmid DNA purified by GenCatch™ Plasmid DNA Midiprep Kit:
DNA Midi Prep

 

  Cat. No.: 2180010 Cat. No.: 2180025
VP1 Buffer 120 ml 265 ml
VP2 Buffer 120 ml 265 ml
VP3 Buffer 120 ml 265 ml
VPN Buffer 225 ml x 2 265 ml x4
VPE Buffer 120 ml 265 ml
RNase A 20 mg/ml 0.60 ml 1.325 ml
GenCatch™ Anion-exchange Column 10 25
GenCatch™ Mini Column 20 50

GenCatch™ Plasmid DNA Maxi Prep Protocol :

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